Ardeshirylajimi ، Abdolreza نويسنده Stem Cell Biology Department, Stem Cell Technology Research Center , , Soleimani، Masoud نويسنده , , Hosseinkhani، Saman نويسنده , , Parivar، Kazem نويسنده Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran , , Yaghmaei، Parichehr نويسنده Islamic Azad University, Science and Research Branch, Tehran, Iran ,
Objective: Human induced pluripotent stem cells (iPSCs) have been shown to have
promising capacity for stem cell therapy and tissue engineering applications. Therefore,
it is essential to compare the ability of these cells with the commonly used mesenchymal
stem cells (MSC) for bone tissue engineering in vitro.
Materials and Methods: In this experimental study, the biological behavior and osteogenic
capacity of the iPSCs were compared with MSCs isolated from human adipose tissue
(AT-MSCs) using 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
assay, Alizarin red staining, alkaline phosphatase (ALP) activity measurements, calcium
content assay and common osteogenic-related genes. Data were reported as the mean ±
SD. One-way analysis of variance (ANOVA) was used to compare the results. A p value of
less than 0.05 was considered statistically significant.
Results: There was a significant difference between the rate of proliferation of the two
types of stem cells; iPSCs showed increased proliferation compared to AT-MSCs. During
osteogenic differentiation, ALP activity and mineralization were demonstrated to be
significantly higher in iPSCs. Although AT-MSCs expressed higher levels of Runx2, iPSCs
expressed higher levels of osteonection and osteocalcin during differentiation.
Conclusion: iPSCs showed a higher capacity for osteogenic differentiation and hold promising
potential for bone tissue engineering and cell therapy applications.