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Title of article :
Pore size distributions of ion exchangers and relation to protein binding capacity
Author/Authors :
Yao، نويسنده , , Yan and Lenhoff، نويسنده , , Abraham M.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
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Abstract :
The pore structure of chromatographic media directly influences macromolecular transport and adsorption, and consequently separation resolution and loading capacity in chromatographic separations. The pore size distribution (PSD) is therefore a central structural characteristic of chromatographic materials and a critical determinant of chromatographic behavior. In this work the PSDs of a set of commercial anion exchangers were determined by inverse size-exclusion chromatography (ISEC). The PSDs were further utilized to develop relations to functional properties of adsorbents, such as intraparticle diffusivity, and static and dynamic binding capacities. We find that the detailed PSD is useful in semi-quantitative understanding of chromatographic behavior. However, more accurate prediction of column behavior requires more thorough knowledge of the pore structure, specifically the connectivity of the pore network, as well as improved understanding of the function of grafted resins.
Keywords :
Ion-exchange chromatography , Static binding capacity , intraparticle diffusion , Dynamic binding capacity , Pore connectivity , Protein chromatography , Inverse size-exclusion chromatography
Journal title :
Journal of Chromatography A
Link To Document :