Record number :
Title of article :
Countercurrent flow of supercritical anti-solvent in the production of pure xanthophylls from Nannochloropsis oculata
Author/Authors :
Cho، نويسنده , , Yueh-Cheng and Wang، نويسنده , , Yuan-Chuen and Shieh، نويسنده , , Chwen-Jen and Lin، نويسنده , , Justin Chun-Te and Chang، نويسنده , , Chieh-Ming J. and Han، نويسنده , , Esther، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
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Abstract :
This study examined pilot scaled elution chromatography coupled with supercritical anti-solvent precipitation (using countercurrent flow) in generating zeaxanthin-rich particulates from a micro-algal species. Ultrasonic agitated acetone extract subjected to column fractionation successfully yielded a fraction containing 349.4 mg/g of zeaxanthin with a recovery of 85%. Subsequently, supercritical anti-solvent (SAS) precipitation of the column fraction at 150 bar and 343 K produced submicron-sized particulates with a concentration of 845.5 mg/g of zeaxanthin with a recovery of 90%. Experimental results from a two-factor response surface method SAS precipitation indicated that purity, mean size and morphology of the precipitates were significantly affected by the flow type configuration, feed flow rate and injection time.
Keywords :
zeaxanthin , Submicron-sized particulates , Column chromatography fractionation , supercritical anti-solvent precipitation
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A
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Link To Document :