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Title of article :
Liquid chromatographic method for the analysis of tocopherols in malt sprouts with supercritical fluid extraction
Author/Authors :
Carlucci، نويسنده , , Giuseppe and Mazzeo، نويسنده , , Pietro and Del Governatore، نويسنده , , Simona and Di Giacomo، نويسنده , , Gabriele and Del Re، نويسنده , , Giovanni، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
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Abstract :
A simple, specific and sensitive high-performance liquid chromatographic method has been developed for the determination of tocopherols in malt sprouts. A supercritical fluid extraction (SFE) procedure was used to isolate tocopherols from the vegetal matrix before quantitative analysis. The analytes were separated on a Zorbax reversed-phase column using methanol–water as mobile phase and quantified by measuring its fluorescence at λem=328 nm after excitation of the analytes at λexc=303 nm. The limits of detection for α-, γ- and δ-tocopherols were 0.04, 0.05, and 0.05 μg/ml, respectively. The calibration graphs of the method were linear from 0.1 to 1.5, 0.2 to 2.5, and 0.2 to 2.0 μg/ml, for α-, γ- and δ-tocopherols, respectively. This SFE and HPLC procedure is simple, precise and accurate for the determination of tocopherols in malt sprouts.
Keywords :
Tocopherols , vitamins
Journal title :
Journal of Chromatography A
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