Record number :
1503419
Title of article :
Effect of liquid chromatography separation of complex matrices on liquid chromatography–tandem mass spectrometry signal suppression
Author/Authors :
Choi، نويسنده , , Bernard K and Hercules، نويسنده , , David M and Gusev، نويسنده , , Arkady I، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Pages :
6
From page :
337
To page :
342
Abstract :
The effect of liquid chromatography separation on liquid chromatography–tandem mass spectrometry (LC–MS–MS) signal response for the characterization of low-molecular-mass compounds in a complex matrix was investigated. Matrix induced signal suppression appears throughout the entire LC–MS–MS analysis of wheat forage extract, with greatest suppression occurring at early retention times. Experimental results show that co-elution of matrix components and analytes from the LC column may be most strongly attributed to column overloading rather than similar analyte and matrix retention behavior. As a result, two-dimensional (LC–LC) separation can be a highly effective approach to address signal suppression effects for the quantitative LC–MS–MS analysis of complex matrix samples.
Keywords :
Hydroxyfenozide , G-Fenozide , Methoxyfenozide
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A
Serial Year :
2001
Link To Document :
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