Record number :
1490538
Title of article :
Fluorescent HPLC for the detection of specific protein oxidation carbonyls – α-aminoadipic and γ-glutamic semialdehydes – in meat systems
Author/Authors :
Utrera، نويسنده , , Mariana and Morcuende، نويسنده , , David and Rodrيguez-Carpena، نويسنده , , Javier-Germلn and Estévez، نويسنده , , Mario، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
7
From page :
500
To page :
506
Abstract :
Precise methodologies for the routine analysis of particular protein carbonyls are required in order to progress in this topic of increasing interest. The present paper originally describes the application of an improved method for the detection of α-aminoadipic and γ-glutamic semialdehydes in a meat system by using a derivatization procedure with p-amino-benzoic acid (ABA) followed by fluorescent high-performance liquid chromatography (HPLC). The method development comprises i) the description of a simple HPLC program which allows the efficient separation of the ABA and the key standard compounds and ii) the optimization of the procedure for the preparation of a meat sample in order to maximize the fluorescent signal for both protein carbonyls. Furthermore, the suitability of this method is evaluated by applying the technique to porcine burger patties. The present procedure enables an accurate and relatively fast analysis of both semialdehydes in meat samples in which they could play an interesting role as reliable indicators of protein oxidation.
Keywords :
?-Aminoadipic semialdehyde , Fluorescent HPLC , ?-Glutamic semialdehyde , Meat systems , Protein oxidation
Journal title :
Meat Science
Serial Year :
2011
Link To Document :
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