Record number :
1487513
Title of article :
Safety evaluation of an α-cyclodextrin glycosyltranferase preparation
Author/Authors :
Bنr، نويسنده , , A and Krul، نويسنده , , C.A.M and Jonker، نويسنده , , D and de Vogel، نويسنده , , N، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
10
From page :
47
To page :
56
Abstract :
Alpha-cyclodextrin glucosyltransferase (α-CGTase, EC 2.4.1.19) is an amylolytic enzyme used for the production of α-cyclodextrin (α-CD), a novel, soluble dietary fiber, from food-grade starch. The safety of an α-CGTase preparation obtained by batch fermentation from a recombinant strain of Escherichia coli K12 harboring the α-CGTase gene from Klebsiella oxytoca strain M5a1 was examined. In a 13-week subchronic toxicity study in rats, the administration by gavage of the α-CGTase preparation at levels of up to 20 ml/kg bw/day, corresponding to a total organic solids dosage of 260 mg/kg bw/day, did not cause any systemic toxic effect. Some signs of irritation were observed in the respiratory tract which occurred, however, in one sex only and/or were not dose-related. Accordingly, these changes were considered to be an unspecific consequence of the reflux and aspiration of the dosing solution. There was no evidence of a genotoxic activity in Ames tests and a chromosome aberration test in cultured human lymphocytes. It is concluded that the examined α-CGTase preparation is safe when used for the production of α-CD.
Journal title :
Regulatory Toxicology and Pharmacology
Journal title :
Regulatory Toxicology and Pharmacology
Serial Year :
2004
Link To Document :
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