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Title of article :
Incidence in diverse pig populations of an IGF2 mutation with potential influence on meat quality and quantity: An assay based on real time PCR (RT-PCR)
Author/Authors :
Carrodeguas، نويسنده , , José Alberto and Burgos، نويسنده , , Carmen and Moreno، نويسنده , , Juan Carlos and Sلnchez-Barragلn، نويسنده , , Ana Cristina and Ventanas، نويسنده , , Sonia and Tarrafeta، نويسنده , , Luis and Barcelona، نويسنده , , José Antonio and Lَpez-Buesa، نويسنده , , Maria Otilia and Oria، نويسنده , , Rosa and Lَpez-Buesa، نويسنده , , Pascual، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
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Abstract :
IGF2, insulin-like growth factor 2, is implicated in myogenesis and lean meat content. A mutation in a single base (A for G substitution) of the gene for IGF2 (position 3072 in intron 3) has been recently described as the cause of a major QTL effect on muscle growth in pigs [Van Laere, A. S, Nguyen, M., Braunschweig, M., Nezer, C., Collete, C., & Moreau, L. et al. (2003). Nature, 425, 832–836]. We describe here a rapid assay based on real time PCR (RT-PCR) to detect this mutation. We have evaluated the incidence of the mutation in commercial pig crosses, in three populations of purebred Iberian or Iberian × Duroc crosses, and in cured meat products and wild boars. The incidence of the mutation varies among these groups. Penetrance of the A mutation is about 80% in the commercial population. Purebred Iberian pigs were all homozygous G/G whereas crosses of Iberian pigs were heterozygous (90%) or homozygous A/A (10%). The implications of this gene for the selection of Iberian pigs are discussed.
Keywords :
Real time PCR , IGF2 , Selection , Iberian pigs
Journal title :
Meat Science
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