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Title of article :
Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry
Author/Authors :
Bhatt، نويسنده , , Mitesh R. Shah، نويسنده , , Sanjay and Shivprakash، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
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Abstract :
A simple, rapid, sensitive and specific ultra performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for the quantification of ethosuximide in human plasma is described. Analyte was chromatographed on a Hypersil Gold C18 column (100 mm × 2.1 mm, i.d., 1.9 μm) with isocratic elution at a flow rate of 0.250 mL/min and pravastatin was used as the internal standard. The assay involves a simple solid-phase extraction procedure of 0.25 mL human plasma and the analysis was performed on a triple-quadrupole tandem mass spectrometer by MRM mode via electrospray ionization (ESI). The method was linear in the concentration range of 0.25–60.0 μg/mL. The lower limit of quantification (LLOQ) was 0.25 μg/mL. The within- and between-day precision and accuracy of the quality control samples were within 10.0%. The recovery was 95.1% and 94.4% for ethosuximide and pravastatin, respectively. The analysis time for each sample was 1.8 min. The method was highly reproducible and gave peaks with excellent chromatography properties.
Keywords :
Ethosuximide , Human plasma , LC–MS/MS , Acquity UPLC
Journal title :
Journal of Chromatography B
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