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Title of article :
Determination of duloxetine in human plasma by liquid chromatography with atmospheric pressure ionization–tandem mass spectrometry and its application to pharmacokinetic study
Author/Authors :
Uttam and Senthamil Selvan، نويسنده , , P. and Gowda، نويسنده , , K. Veeran and Mandal، نويسنده , , U. and Sam Solomon، نويسنده , , W.D. and Pal، نويسنده , , T.K.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
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Abstract :
A rapid, sensitive and accurate liquid chromatographic–tandem mass spectrometry (LC–MS–MS) method is described for the determination of duloxetine in human plasma. Duloxetine was extracted from plasma using methanol and separated on a C18 column. The mobile phase consisting of a mixture of acetonitrile and 5 mM ammonium acetate (45:55, v/v, pH 3.5) was delivered at a flow rate of 0.3 ml/min. Atmospheric pressure ionization (API) source was operated in positive ion mode. Multiple reaction monitoring (MRM) mode using the transitions of m/z 298.1 → m/z 44.0 and m/z 376.2 → m/z 123.2 were used to quantify duloxetine and internal standard (I.S.), respectively. The linearity was obtained over the concentration range of 0.1–50.0 ng/ml and the lower limit of quantitation (LLOQ) was 0.1 ng/ml. This method was successfully applied to pharmacokinetic study of a duloxetine formulation product after oral administration to healthy human subjects.
Keywords :
Duloxetine , LC-API–MS–MS
Journal title :
Journal of Chromatography B
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