Record number :
Title of article :
Quantitative determination of latrunculins A and B in the Red Sea sponge Negombata magnifica by high performance liquid chromatography
Author/Authors :
Khalifa، نويسنده , , Sherief and Ahmed، نويسنده , , Safwat and Mesbah، نويسنده , , Mostafa and Youssef، نويسنده , , Diaa and Hamann، نويسنده , , Mark، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
Pages :
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Abstract :
An accurate, reproducible and sensitive method for the quantitative determination of latrunculins A and B in the organic extract of the Red Sea sponge Negombata magnifica was developed and validated. Latrunculin A and B concentrations were determined by RP-C18-HPLC and a mobile phase consisting of acetonitrile and water (60:40, v/v). The flow rate utilized was 1 mL min−1 and the detector was set at 235 nm. The HPLC analysis of several N. magnifica samples collected from different locations in the Red Sea revealed that Ras Mohamed had the highest concentrations of latrunculin A, while Safaga had the highest levels of latrunculin B. Also, a comparison between latrunculin concentrations in the summer and winter revealed that the yield of latrunculins were generally higher in the winter.
Keywords :
Latrunculin A , Season , Latrunculin B , High Performance Liquid Chromatography , location , Negombata magnifica , Red Sea
Journal title :
Journal of Chromatography B
Link To Document :