Record number :
1456033
Title of article :
Determination of nicotine and its metabolites in urine by solid-phase extraction and sample stacking capillary electrophoresis-mass spectrometry
Author/Authors :
Baidoo، نويسنده , , Edward E.K. and Clench، نويسنده , , Malcolm R. and Smith، نويسنده , , Robert F. and Tetler، نويسنده , , Lee W.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Pages :
11
From page :
303
To page :
313
Abstract :
The combination of capillary electrophoresis (CE) and mass spectrometry (MS) with solid-phase extraction (SPE) has been used for the identification of nicotine and eight of its metabolites in urine. The recovery of cotinine from cotinine-spiked urine, by C18 SPE, was found to be 98%. Smokers urine (200 ml) was preconcentrated 200-fold via SPE prior to analysis. The sample stacking mode of CE, when compared to capillary zone electrophoresis, was shown to improve peak efficiency by 132-fold. The combination of hydrodynamic and electrokinetic injection was studied with sample stacking/CE/MS. The on-column limits of detection (LOD) of nicotine and cotinine, by this technique, were found to be 0.11 and 2.25 μg/ml, respectively. Hence, LODs of nicotine and cotinine in urine after 200-fold preconcentration were 0.55 and 11.25 ng/ml, respectively.
Keywords :
Nicotinemetabolites , Sample stacking
Journal title :
Journal of Chromatography B
Link To Document :