Record number :
Title of article :
Determination of the major mercapturic acids of 1,3-butadiene in human and rat urine using liquid chromatography with tandem mass spectrometry
Author/Authors :
Urban، نويسنده , , Michael and Gilch، نويسنده , , Gerhard and Schepers، نويسنده , , Georg and Miert، نويسنده , , Erik van and Scherer، نويسنده , , Gerhard، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Pages :
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Abstract :
The major urinary metabolites of 1,3-butadiene are monohydroxybutenyl-mercapturic acids (MHBMA) and dihydroxy-butyl-mercapturic acid (DHBMA). These metabolites can be used as biomarkers of exposure to this diene. In order to determine the smoking-related exposure to 1,3-butadiene, we have developed a rapid LC-MS/MS method for the determination of MHBMA and DHBMA in urine of humans and rats. The method requires 2–5 ml of urine which is solid phase extracted prior to LC-MS/MS analysis. Precision for MHBMA is ≤11.2% for human and ≤17% for rat urine. Corresponding values for DHBMA are ≤7.2 and ≤19%, respectively. Recovery rates are approximately 100% for both analytes in human urine and about 115% in rat urine. Limits of detection (LOD) are for humans 0.9 and 23 ng/ml and for rats 1.5 and 33 ng/ml for MHBMA and DHBMA, respectively. Application of the method to urine of humans and rats showed a significant effect of tobacco smoke exposure on the urinary excretion of MHBMA and the metabolic ratio DHBMA/(DHBMA + MHBMA).
Keywords :
1 , Mercapturic acids , 3-Butadiene
Journal title :
Journal of Chromatography B
Link To Document :