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Title of article :
Protein mapping in rat basophilic leukaemia cells
Author/Authors :
Carroll، نويسنده , , K.M. and Carey، نويسنده , , E.M. and Helm، نويسنده , , B.A.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
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Abstract :
This review discusses some of our recent studies on rat basophilic leukaemia (RBL) cells, a model cell line for mast cell function. Our interest in these cells is a consequence of the role played by mast cells in the allergic response. Thus far we have described the identification of several spots, and their pI/Mr co-ordinates. Here we describe how we can further decipher the mast cell proteome using a variety of staining/immuno-blotting procedures. We demonstrate the sensitivity of staining procedures and immuno-blotting using an anti-phosphotyrosine antibody. Our aim is to contribute to the ever-expanding two-dimensional gel and phosphoprotein databases currently available.
Journal title :
Journal of Chromatography B
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