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Title of article :
Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production
Author/Authors :
Anita R. Bhandiwad، نويسنده , , Ashwini and Shaw، نويسنده , , A. Joe and Guss، نويسنده , , Adam and Guseva، نويسنده , , Anna and Bahl، نويسنده , , Hubert and Lynd، نويسنده , , Lee R.، نويسنده ,
Issue Information :
دوماهنامه با شماره پیاپی سال 2014
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Abstract :
The thermophilic anaerobe Thermoanaerobacterium saccharolyticum JW/SL-YS485 was investigated as a host for n-butanol production. A systematic approach was taken to demonstrate functionality of heterologous components of the clostridial n-butanol pathway via gene expression and enzymatic activity assays in this organism. Subsequently, integration of the entire pathway in the wild-type strain resulted in n-butanol production of 0.85 g/L from 10 g/L xylose, corresponding to 21% of the theoretical maximum yield. We were unable to integrate the n-butanol pathway in strains lacking the ability to produce acetate, despite the theoretical overall redox neutrality of n-butanol formation. However, integration of the n-butanol pathway in lactate deficient strains resulted in n-butanol production of 1.05 g/L from 10 g/L xylose, corresponding to 26% of the theoretical maximum.
Keywords :
Metabolic engineering , biofuels , n-Butanol , thermophiles
Journal title :
Metabolic Engineering
Link To Document :