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Title of article :
Dynamic modeling of aerobic growth of Shewanella oneidensis. Predicting triauxic growth, flux distributions, and energy requirement for growth
Author/Authors :
Song، نويسنده , , Hyun-Seob and Ramkrishna، نويسنده , , Doraiswami and Pinchuk، نويسنده , , Grigoriy E. and Beliaev، نويسنده , , Alexander S. and Konopka، نويسنده , , Allan E. and Fredrickson، نويسنده , , James K.، نويسنده ,
Issue Information :
دوماهنامه با شماره پیاپی سال 2013
Pages :
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Abstract :
A model-based analysis is conducted to investigate metabolism of Shewanella oneidensis MR-1 strain in aerobic batch culture, which exhibits an intriguing growth pattern by sequentially consuming substrate (i.e., lactate) and by-products (i.e., pyruvate and acetate). A general protocol is presented for developing a detailed network-based dynamic model for S. oneidensis based on the Lumped Hybrid Cybernetic Model (L-HCM) framework. The L-HCM, although developed from only limited data, is shown to accurately reproduce exacting dynamic metabolic shifts, and provide reasonable estimates of energy requirement for growth. Flux distributions in S. oneidensis predicted by the L-HCM compare very favorably with 13C-metabolic flux analysis results reported in the literature. Predictive accuracy is enhanced by incorporating measurements of only a few intracellular fluxes, in addition to extracellular metabolites. The L-HCM developed here for S. oneidensis is consequently a promising tool for the analysis of intracellular flux distribution and metabolic engineering.
Keywords :
Shewanella oneidensis , Dynamic metabolic model , 13C-MFA , ATP requirement for growth , Lumped hybrid cybernetic model (L-HCM)
Journal title :
Metabolic Engineering
Link To Document :