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Title of article :
Chlorogenic acid, a dietary polyphenol, protects acetaminophen-induced liver injury and its mechanism
Author/Authors :
LiLi Ji، نويسنده , , Ping Jiang، نويسنده , , Bin Lu، نويسنده , , Yuchen Sheng، نويسنده , , Xin Wang، نويسنده , , Zhengtao Wang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
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Abstract :
Chlorogenic acid (CGA) is one of the most abundant dietary polyphenols, possessing well-known antioxidant capacity. The present study is designed to observe the protection provided by CGA against acetaminophen (AP)-induced liver injury in mice in vivo and the underlying mechanisms engaged in this process. Serum transaminases analysis and liver histological evaluation demonstrated the protection of CGA against AP-induced liver injury. CGA treatment decreased the increased number of liver apoptotic cells induced by AP in a dose-dependent manner. CGA also inhibited AP-induced cleaved activation of caspase-3, 7. Moreover, CGA reversed AP-decreased liver reduced glutathione (GSH) levels, glutamate-cysteine ligase (GCL) and glutathione reductase activity. Further results showed that CGA increased mRNA and protein expression of the catalytic subunit of GCL (GCLC), thioredoxin (Trx) 1/2 and thioredoxin reductase (TrxR) 1. Furthermore, CGA abrogated AP-induced phospholyated activation of ERK1/2, c-Jun N-terminal kinase (JNK), p38 kinases and molecular signals upstream. The results of this study demonstrate that CGA counteracts AP-induced liver injury at various levels by preventing apoptosis and oxidative stress damage, and more specifically, both the GSH and Trx antioxidant systems and the mitogen-activated protein kinase (MAPK) signaling cascade appear to be engaged in this protective mechanism.
Keywords :
MAPK , Chlorogenic acid , Acetaminophen , Caspase , GSH , Trx
Journal title :
The Journal of Nutritional Biochemistry
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