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Title of article :
Protective effects of dietary EPA and DHA on ischemia–reperfusion-induced intestinal stress
Author/Authors :
Viral Brahmbhatt، نويسنده , , Manuel Oliveira، نويسنده , , Muriel Briand، نويسنده , , Geneviève Perrisseau، نويسنده , , Viktoria Bastic Schmid، نويسنده , , Frédéric Destaillats، نويسنده , , Cecil Pace-Asciak، نويسنده , , Jalil Benyacoub ، نويسنده , , Nabil Bosco، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
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Abstract :
The immunoregulatory effects of dietary omega-3 fatty acids are still not fully characterized. The aim of this study was to determine whether dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) intake limits intestinal ischemia–reperfusion (IR) injury. To test this, rats were fed either control or EPA/DHA supplemented diet for 3 weeks following which they underwent either a sham or an IR surgical protocol. A significant reduction in mucosal damage was observed after EPA/DHA supplemented diet as reflected by maintenance of total protein content. To address the underlying mechanisms of protection, we measured parameters of oxidative stress, intestinal and serological cytokines and intestinal eicosanoids. Interestingly, EPA/DHA fed animals displayed a higher activity of oxidative stress enzyme machinery, i.e., superoxide dismutase and catalase in addition to a reduction in total nitrate/nitrite content. While no changes in cytokines were observed, eicosanoid analyses of intestinal tissue revealed an increase in metabolites of the 12-lipoxygenase pathway following IR. Further, IR in EPA/DHA fed animals was accompanied by a significant increase of 17,18-epoxyeicosatetraenoic acid, 8-Iso prostaglandin F3α and thromboxane B3, by more than 12-, 6-, 3-fold, respectively. Thus, the data indicate that EPA/DHA supplementation may be able to reduce early intestinal IR injury by anti-oxidative and anti-inflammatory mechanisms.
Keywords :
Omega-3 , eicosanoids , oxidative stress , Docosahexaenoic acid , Nutrition , Ischemia-reperfusion , Eicosapentaenoic acid
Journal title :
The Journal of Nutritional Biochemistry
Link To Document :