Record number :
Title of article :
Cloning and expression of metallothionein mutant α-KKS-α in Anabaena sp. PCC 7120
Author/Authors :
Qiang Shao، نويسنده , , Dingji Shi، نويسنده , , Fu-Ying Hao، نويسنده , , Lina Ma، نويسنده , , Zhen-Jia Chen، نويسنده , , Mei-Min Yu، نويسنده , , Bing-gen Ru، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
Pages :
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Abstract :
The mouse metallothionein (mMT) mutant α-KKS-α has a higher capacity for binding heavy metals than wild type mMT. The mMT mutant α-KKS-α gene was placed under the control of the strong promoter PpbsA to generate the intermediate vector pRL-α-KKS-α. pRLα-KKS-α was then linked with the plasmid pDC-08 to construct shuttle expression vector pDC-αKKS-α. This expression vector was transformed into Anabaena sp. PCC 7120 using triparental conjugative transfer. After antibiotic selection (ampicillin and kanamycin), transgenic Anabaena was identified by PCR and Western blotting. The expression level of the mMT mutation α-KKS-α reached 7.4 mg/g dry cells weight, as detected by ELISA, and heavy metal resistance of the transgenic Anabaena was significantly improved.
Keywords :
Triparental conjugative transfer , Heavy metal resistance , Metallothionein mutation , Transgenic cyanobacteria
Journal title :
Marine Pollution Bulletin
Link To Document :