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Title of article :
Localization of the N-Terminus of Minor Coat Protein IIIa in the Adenovirus Capsid
Author/Authors :
Carmen San Martin، نويسنده , , Joel N. Glasgow، نويسنده , , Anton Borovjagin، نويسنده , , Matthew S. Beatty، نويسنده , , Elena A. Kashentseva، نويسنده , , David T. Curiel، نويسنده , , Roberto Marabini، نويسنده , , Igor P. Dmitriev، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
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Abstract :
Minor coat protein IIIa is conserved in all adenoviruses (Ads) and is required for correct viral assembly, but its precise function in capsid organization is unknown. The latest Ad capsid model proposes that IIIa is located underneath the vertex region. To obtain experimental evidence on the location of IIIa and to further define its role, we engineered the IIIa gene to encode heterologous N-terminal peptide extensions. Recombinant Ad variants with IIIa encoding six-histidine (6His) tag, 6His, and FLAG peptides, or with 6His linked to FLAG with a (Gly4Ser)3 linker were rescued and analyzed for virus yield, capsid incorporation of heterologous peptides, and capsid stability. Longer extensions could not be rescued. Western blot analysis confirmed that the modified IIIa proteins were expressed in infected cells and incorporated into virions. In the Ad encoding the 6His–linker–FLAG–IIIa gene, the 6His tag was present in light particles, but not in mature virions. Immunoelectron microscopy of this virus showed that the FLAG epitope is not accessible to antibodies on the viral particles. Three-dimensional electron microscopy and difference mapping located the IIIa N-terminal extension beneath the vertex complex, wedged at the interface between the penton base and peripentonal hexons, therefore supporting the latest proposed model. The position of the IIIa N-terminus and its low tolerance for modification provide new clues for understanding the role of this minor coat protein in Ad capsid assembly and disassembly.
Keywords :
polypeptide IIIa , adenovirus , VIRUS STRUCTURE , minor coat protein , three-dimensional electron microscopy
Journal title :
Journal of Molecular Biology
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