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Title of article :
Crystal Structure of MshB from Mycobacterium tuberculosis, a Deacetylase Involved in Mycothiol Biosynthesis
Author/Authors :
Andrew A. McCarthy، نويسنده , , Neil A. Peterson، نويسنده , , Rainer Knijff، نويسنده , , Edward N. Baker، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
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Abstract :
All living species require protection against the damaging effects of the reactive oxygen species that are a natural by-product of aerobic life. In most organisms, glutathione is a critical component of these defences, maintaining a reducing environment inside cells. Some bacteria, however, including pathogenic mycobacteria, use an alternative low molecular mass thiol compound called mycothiol (MSH) for this purpose. Enzymes that synthesize MSH are attractive candidates for the design of novel anti-TB drugs because of the importance of MSH for mycobacterial life and the absence of such enzymes in humans. We have determined the three-dimensional structure of MshB (Rv1170), a metal-dependent deacetylase from Mycobacterium tuberculosis that catalyses the second step in MSH biosynthesis. The structure, determined at 1.9 Å resolution by X-ray crystallography (R=19.0%, Rfree=21.4%), reveals an α/β fold in which helices pack against a seven-stranded mostly parallel β-sheet. Large loops emanating from the C termini of the β-strands enclose a deep cavity, which is the location of the putative active site. At the bottom of this cavity is a metal-binding site associated with a sequence motif AHPDDE that is invariant in all homologues. An adventitiously bound β-octylglucoside molecule, used in crystallization, enables us to model the binding of the true substrate and propose a metal-dependent mechanistic model for deacetylation. Sequence comparisons indicate that MshB is representative of a wider family of enzymes that act on substituted N-acetylglucosamine residues, including a deacetylase involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors in eukaryotes.
Keywords :
deacetylase , Tuberculosis , crystal structure , enzyme mechanism , mycothiol biosynthesis
Journal title :
Journal of Molecular Biology
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