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Title of article :
Crystal Structures of Aspergillus oryzae Aspartic Proteinase and its Complex with an Inhibitor Pepstatin at 1.9 Å Resolution
Author/Authors :
Shigehiro Kamitori، نويسنده , , Akashi Ohtaki، نويسنده , , Hironori Ino، نويسنده , , Michio Takeuchi، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
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Abstract :
The X-ray structures of Aspergillus oryzae aspartic proteinase (AOAP) and its complex with inhibitor pepstatin have been determined at 1.9 Å resolution. AOAP was crystallized in an orthorhombic system with the space group P212121 and cell dimensions of a=49.4 Å, b=79.4 Å, and c=93.6 Å. By the soaking of pepstatin, crystals are transformed into a monoclinic system with the space group C2 and cell dimensions of a=106.8 Å, b=38.6 Å, c=78.7 Å, and β=120.3°. The structures of AOAP and AOAP/pepstatin complex were refined to an R-factor of 0.177 (Rfree=0.213) and of 0.185 (0.221), respectively. AOAP has a crescent-shaped structure with two lobes (N-lobe and C-lobe) and the deep active site cleft is constructed between them. At the center of the active site cleft, two Asp residues (Asp33 and Asp214) form the active dyad with a hydrogen bonding solvent molecule between them. Pepstatin binds to the active site cleft via hydrogen bonds and hydrophobic interactions with the enzyme. The structures of AOAP and AOAP/pepstatin complex including interactions between the enzyme and pepstatin are very similar to those of other structure-solved aspartic proteinases and their complexes with pepstatin. Generally, aspartic proteinases cleave a peptide bond between hydrophobic amino acid residues, but AOAP can also recognize the Lys/Arg residue as well as hydrophobic amino acid residues, leading to the activation of trypsinogen and chymotrypsinogen. The X-ray structure of AOAP/pepstatin complex and preliminary modeling show two possible sites of recognition for the positively charged groups of Lys/Arg residues around the active site of AOAP.
Keywords :
aspartic proteinase , pepstatin , trypsinogen activation , Aspergillus oryzae , X-ray structure
Journal title :
Journal of Molecular Biology
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