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Title of article :
A composite enzymatic membrane for the specific separation and concentration of l glycerophosphate from a racemic d/l glycerophosphate mixture
Author/Authors :
Bernard Perrin، نويسنده , , Koffi Fiaty، نويسنده , , Catherine Charcosset، نويسنده , , Hichem Moueddeb، نويسنده , , Roger Couturier، نويسنده , , Bernard Maïsterrena، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Pages :
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Abstract :
A composite enzymatic membrane (CEM), made up of two enzymatic membranes carrying two enzymes catalyzing two opposite reactions on either side of a permeable negatively charged membrane was used in this study. The phosphatase/kinase enzyme couple was chosen to make CEM permitting the active and selective transport of small-phosphorylated molecules. Here, we show that a CEM involving the alkaline phosphatase/glycerokinase enzyme couple permits, in a compartmentalized system, not only the separation of the d and l forms of a racemic mixture of (d/l) glycerophosphate, but also the simultaneous concentration of the l form alone.
Keywords :
Enzymatic membranes , Immobilized enzymes , Racemic separation , Active transports
Journal title :
Enzyme and Microbial Technology
Serial Year :
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