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Title of article :
Cloning, expression, partial characterization and structural modeling of a novel esterase from Pyrococcus furiosus
Author/Authors :
Rodrigo Volcan Almeida، نويسنده , , Sylvia Maria Campbell Alquéres، نويسنده , , Ariane Leites Larentis، نويسنده , , Shaila C?ntia R?ssle، نويسنده , , Alexander Machado Cardoso، نويسنده , , Welington In?cio Almeida، نويسنده , , Paulo Mascarello Bisch، نويسنده , , Tito Livio Moitinho Alves، نويسنده , , Orlando Bonif?cio Martins، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
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Abstract :
In this report the ORF PF2001 from the hyperthermophilic archaeon Pyrococcus furiosus was identified and its protein sequence was characterized in silico, revealing characteristics of the conserved domains of the dipeptidyl aminopeptidases, hydrolases of the α/β superfamily, esterases and lipases. In order to understand its function, the ORF PF2001 without the 60 bp of the 5′-terminus, responsible for encoding a signal peptide (PF2001Δ60), was cloned and expressed in Escherichia coli BL21(DE3) pLysS, and the recombinant enzyme was characterized for esterase, lipase and protease activities. The total protein extract from E. coli harboring the plasmid containing the ORF PF2001Δ60 exhibited its highest activity towards the substrate 4-methylumbelliferyl-heptanoate (C7) and lower activities towards 4-methylumbelliferyl-acetate (C2) and 4-methylumbelliferyl-palmitate (C16). The enzyme was thermostable for 120 min at 75 °C and was completely inhibited by 1 mM PMSF. A theoretical structural model was constructed by comparative modeling using as template a prolyl oligopeptidase from Sus scrofa. Although no protease activity was detected a putative catalytic triad (Ser149, Asp233 and His264) with high similarity to the template was identified. The structural characteristics that confer enzymatic specificity to the P. furiosus enzyme are discussed. Taken together the data strongly suggest that ORF PF2001 from P. furiosus is responsible for encoding a novel esterase.
Keywords :
Esterases , Lipases , Proteases , Comparative modeling , Extremozymes , Thermophilic enzymes , Archaea , homology modeling
Journal title :
Enzyme and Microbial Technology
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