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Title of article :
A modular microfluidic system for deoxyribonucleic acid identification by short tandem repeat analysis Original Research Article
Author/Authors :
Carmen R. Reedy، نويسنده , , Kristin A. Hagan، نويسنده , , Daniel J. Marchiarullo، نويسنده , , Alison H. Dewald، نويسنده , , Annalise Barron، نويسنده , , Joan M. Bienvenue، نويسنده , , James P. Landers، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
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Abstract :
Microfluidic technology has been utilized in the development of a modular system for DNA identification through STR (short tandem repeat) analysis, reducing the total analysis time from the ∼6 h required with conventional approaches to less than 3 h. Results demonstrate the utilization of microfluidic devices for the purification, amplification, separation and detection of 9 loci associated with a commercially-available miniSTR amplification kit commonly used in the forensic community. First, DNA from buccal swabs purified in a microdevice was proven amplifiable for the 9 miniSTR loci via infrared (IR)-mediated PCR (polymerase chain reaction) on a microdevice. Microchip electrophoresis (ME) was then demonstrated as an effective method for the separation and detection of the chip-purified and chip-amplified DNA with results equivalent to those obtained using conventional separation methods on an ABI 310 Genetic Analyzer. The 3-chip system presented here demonstrates development of a modular, microfluidic system for STR analysis, allowing for user-discretion as to how to proceed after each process during the analysis of forensic casework samples.
Keywords :
Microfluidics , Short tandem repeat analysis , Deoxyribonucleic acid identification , Forensics
Journal title :
Analytica Chimica Acta
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