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Title of article :
8-Cyclopentadienyltricarbonyl 99mTc 8-Oxooctanoic Acid: A Novel Radiotracer for Evaluation of Medium Chain Fatty Acid Metabolism in the Liver
Author/Authors :
Choe، Yearn Seong نويسنده , , Chi، Dae Yoon نويسنده , , Lee، Kyung-Han نويسنده , , Choi، Yong نويسنده , , Kim، Byung-Tae نويسنده , , Lee، Byung Chul نويسنده , , Paik، Jin-Young نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
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Abstract :
8-Cyclopentadienyltricarbonyl 99mTc 8-oxooctanoic acid (99mTc-CpTTOA; 1a) was synthesized for evaluation of medium chain fatty acid metabolism in the liver. 99mTc-CpTTOA was prepared in high radiochemical yield (50-63%) by a double ligand transfer reaction of methyl 8-ferrocenyl-8oxooctanoate and Na99mTcO4 in the presence of CrCl3 and Cr(CO)6, followed by hydrolysis. This radiotracer was shown to be stable (>90% at 6 h) when incubated with human serum. Aqueous extraction of the radioactivity from the liver and blood samples of mice suggested that 99mTcCpTTOA was mainly metabolized via (beta)-oxidation in the liver, and the radioactivity was retained longer in CCl4-treated mice than in control mice, possibly due to impaired (beta)-oxidation in the former. Planar images of rats injected with 99mTc-CpTTOA showed accumulation of the radioactivity in the liver, kidneys, and bladder with rapid hepatic clearance as a function of time. Analysis of the metabolites from the liver and urine samples of rats further supported that 99mTc-CpTTOA was metabolized to 4-cyclopentadienyltricarbonyl 99mTc 4-oxobutanoic acid (99mTc-CpTTBA; 1c) via (beta)oxidation. The results suggested that this radiotracer might be of valuable use in the evaluation of fatty acid metabolism in the liver.
Keywords :
Cretan Mediterranean diet , folate , Ischaemic heart disease , homocysteine
Journal title :
Bioconjugate Chemistry
Journal title :
Bioconjugate Chemistry
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