Record number :
1019424
Title of article :
Intramolecular Strain Coordinates Kinesin Stepping Behavior along Microtubules
Author/Authors :
Ahmet Yildiz، نويسنده , , Michio Tomishige، نويسنده , , Arne Gennerich، نويسنده , , Ronald D. Vale، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2008
Pages :
12
From page :
1030
To page :
1041
Abstract :
Kinesin advances 8 nm along a microtubule per ATP hydrolyzed, but the mechanism responsible for coordinating the enzymatic cycles of kinesinʹs two identical motor domains remains unresolved. Here, we have tested whether such coordination is mediated by intramolecular tension generated by the “neck linkers,” mechanical elements that span between the motor domains. When tension is reduced by extending the neck linkers with artificial peptides, the coupling between ATP hydrolysis and forward stepping is impaired and motorʹs velocity decreases as a consequence. However, speed recovers to nearly normal levels when external tension is applied by an optical trap. Remarkably, external load also induces bidirectional stepping of an immotile kinesin that lacks its mechanical element (neck linker) and fuel (ATP). Our results indicate that the kinesin motor domain senses and responds to strain in a manner that facilitates its plus-end-directed stepping and communication between its two motor domains.
Journal title :
CELL
Serial Year :
2008
Link To Document :
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