Record number :
101832
Title of article :
Recent advances in biomolecular process intensification
Author/Authors :
Choe، Woo-seok نويسنده , , Nian، Rui نويسنده , , Lai، Wen-Bin نويسنده ,
Issue Information :
دوهفته نامه با شماره پیاپی سال 2006
Pages :
-885
From page :
886
To page :
0
Abstract :
Escherichia coli has been the most popular cellular biofactory for the production of many biomolecules including recombinant proteins whose bioactivities are not dependent on complicated post-translational modifications such as glycosylation. Despite many advantages of protein production using E. coli, the formation of insoluble inclusion bodies (IBs) poses a major challenge to harnessing E. coli as a host of choice. Furthermore, the complicated processing steps associated with IB extraction, solubilisation of IBs and refolding of the solubilised-denatured IB proteins, which are typically encountered in the traditional IB processing flowsheet, significantly compromise process economics of IB-route protein production using E. coli. In this paper, many recent advances in innovative IB processing technologies are reviewed with a special emphasis on their potential to contribute to process intensification, which is critical to achieve better process economics in the IB-route recombinant protein production using E. coli.
Keywords :
Process intensification , inclusion body , E. coli
Journal title :
CHEMICAL ENGINEERING SCIENCE
Journal title :
CHEMICAL ENGINEERING SCIENCE
Serial Year :
2006
Link To Document :
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