Record number :
Title of article :
Force spectroscopy of covalent bond rupture versus protein extraction
Author/Authors :
Rehana Afrin، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
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Abstract :
Development of protein extraction and identification methods from a live cell surface using minimally invasive technology has an important implication as a possible tool to study time-dependent changes of the distribution of intrinsic membrane proteins in specific locals on the cell membrane.We have approached to this problem using an atomic force microscope mounted with a chemically modified probe with amino reactive covalent crosslinkers against amino groups on the membrane proteins.We discuss the probability of protein extraction versus covalent bond rupture in the experimentally observed rupture force in protein extraction. Possibility of protein unfolding by mechanical stretching during extraction from the cell surface is discussed
Keywords :
membrane protein , Atomic force microscope , Protein stretching , covalent bond , Nano-mechanics
Journal title :
Applied Surface Science
Journal title :
Applied Surface Science
Serial Year :
Link To Document :